GSM4437093: 3T9 MycER RA ER; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Esr1
Cell type
Cell type Class
Others
Cell type
Fibroblasts
NA
NA
Attributes by original data submitter
Sample
source_name
immortalized fibroblasts
cell type
3T9 fibroblastst
genotype
infected with BP-MycER-RA
treatment
OHT 4h
chip antibody
ER-alpha 06-935 Santa Cruz
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 250 bp were immunoprecipitated using different antibodies. Libraries were prepared for Illumina sequencing using a standard protocol consisting in blunting, addition of dA overhangs, ligation of Illumina adapters, selection with AMPure beads and PCR with index primers.